The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain
Abstract: The blue biliprotein phycocyanin, produced by photo‐autotrophic cyanobacteria including spirulina (Arthrospira) and marketed as a natural food supplement or “nutraceutical,” is reported to have anti‐inflammatory, antioxidant, immunomodulatory, and anticancer activity. These diverse biological activities have been specifically attributed to the phycocyanin chromophore, phycocyanobilin (PCB). However, the mechanism of action of PCB and the molecular targets responsible for the beneficial properties of PCB are not well understood. We have developed a procedure to rapidly cleave the PCB pigment from phycocyanin by ethanolysis and then characterized it as an electrophilic natural product that interacts covalently with thiol nucleophiles but lacks any appreciable cytotoxicity or antibacterial activity against common pathogens and gut microbes. We then designed alkyne‐bearing PCB probes for use in chemical proteomics target deconvolution studies. Target identification and validation revealed the cysteine protease legumain (also known as asparaginyl endopeptidase, AEP) to be a target of PCB. Inhibition of this target may account for PCB's diverse reported biological activities.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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The Cyanobacterial “Nutraceutical” Phycocyanobilin Inhibits Cysteine Protease Legumain ; day:26 ; month:01 ; year:2023 ; extent:11
ChemBioChem ; (26.01.2023) (gesamt 11)
- Urheber
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Wilkinson, Isabel V. L.
Castro‐Falcón, Gabriel
Roda‐Serrat, Maria C.
Purdy, Trevor N.
Straetener, Jan
Brauny, Melanie M.
Maier, Lisa
Brötz, Heike
Christensen, Lars P.
Sieber, Stephan A.
Hughes, Chambers C.
- DOI
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10.1002/cbic.202200455
- URN
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urn:nbn:de:101:1-2023012714263282448319
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:32 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Wilkinson, Isabel V. L.
- Castro‐Falcón, Gabriel
- Roda‐Serrat, Maria C.
- Purdy, Trevor N.
- Straetener, Jan
- Brauny, Melanie M.
- Maier, Lisa
- Brötz, Heike
- Christensen, Lars P.
- Sieber, Stephan A.
- Hughes, Chambers C.
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