Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway
Abstract: Gestational diabetes mellitus (GDM), defined as glucose intolerance occurring or first detected during pregnancy, affects approximately 8% of pregnancies worldwide. The dysfunction of trophoblasts in pregnancies complicated by GDM is associated with changes in trophoblast cell functions, resulting in compromised proliferation and regulation of the cell cycle. Cyclin B1 (CCNB1), a pivotal controller of the start of mitosis, is crucial in these mechanisms. Nevertheless, the precise function of CCNB1 in trophoblast dysfunction related to GDM has not been extensively investigated. The aim of this study was to investigate CCNB1’s role in high glucose (HG)-triggered trophoblast. Herein, we revealed that in HG-stimulated HTR8/SVneo cells, CCNB1 is highly expressed. Knockdown of CCNB1 significantly promotes the growth of HG-stimulated HTR8/SVneo cells and suppresses inflammation (p < 0.05). Additionally, reducing CCNB1 expression significantly improves glucose uptake and inhibits the Wnt/β-catenin pathway in HG-stimulated HTR8/SVneo cells (p < 0.05). In conclusion, our study demonstrated that the deletion of CCNB1 can alleviate trophoblast dysfunction induced by HG in GDM through the Wnt/β-catenin pathway. This suggests that CCNB1 may be a potential target for managing GDM. Although our results underscore the potential therapeutic benefits of reducing CCNB1 in mitigating trophoblast dysfunction, it is important to note that the study is limited by its reliance on a single cell line and the absence of in vivo validation.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway ; volume:20 ; number:1 ; year:2025 ; extent:9
Open medicine ; 20, Heft 1 (2025) (gesamt 9)
- Urheber
-
Xiao, Biru
Zhang, Wenmiao
Ji, Nini
Chen, Qiuyue
- DOI
-
10.1515/med-2024-1119
- URN
-
urn:nbn:de:101:1-2501140623067.549185178659
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:30 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Xiao, Biru
- Zhang, Wenmiao
- Ji, Nini
- Chen, Qiuyue
Ähnliche Objekte (12)
Knockdown of Aurora kinase B alleviates high glucose-triggered trophoblast cells damage and inflammation during gestational diabetes
RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway
Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis
FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior
Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway
Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal
Knockdown of SDC-1 Gene Alleviates the Metabolic Pathway for the Development of MODS
USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway
Knockdown of USP7 alleviates atherosclerosis in ApoE-deficient mice by regulating EZH2 expression
ClC-2 knockdown prevents cerebrovascular remodeling via inhibition of the Wnt/β-catenin signaling pathway
Resolvin D1 alleviates apoptosis triggered by endoplasmic reticulum stress in IPEC-J2 cells
Knockdown of Aurora kinase B alleviates high glucose-triggered trophoblast cells damage and inflammation during gestational diabetes
RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway
Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis
FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior
Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway
Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal
Knockdown of SDC-1 Gene Alleviates the Metabolic Pathway for the Development of MODS
USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway
Knockdown of USP7 alleviates atherosclerosis in ApoE-deficient mice by regulating EZH2 expression
ClC-2 knockdown prevents cerebrovascular remodeling via inhibition of the Wnt/β-catenin signaling pathway
Resolvin D1 alleviates apoptosis triggered by endoplasmic reticulum stress in IPEC-J2 cells
Knockdown of Aurora kinase B alleviates high glucose-triggered trophoblast cells damage and inflammation during gestational diabetes
RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway
Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis
FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior
Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway
Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal
Knockdown of SDC-1 Gene Alleviates the Metabolic Pathway for the Development of MODS
USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway
Knockdown of USP7 alleviates atherosclerosis in ApoE-deficient mice by regulating EZH2 expression
ClC-2 knockdown prevents cerebrovascular remodeling via inhibition of the Wnt/β-catenin signaling pathway