Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)‐releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly (vinyl alcohol) fiber mats bearing poly (lactic‐co‐glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six‐layered cardiomyocyte sheets are transplanted with a VEGF‐releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF‐releasing mat, and incorporate larger‐diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF‐releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF‐releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets ; volume:17 ; number:8 ; year:2017 ; extent:8 ; Macromolecular bioscience ; 17, Heft 8 (2017) (gesamt 8)

Contributors, Places and Time

• Creator: Nagase, Kenichi ; Nagumo, Yuhei ; Kim, Miri ; Kim, Hee‐Jung ; Kyung, Hei‐Won ; Chung, Hye‐Jin ; Sekine, Hidekazu ; Shimizu, Tatsuya ; Kanazawa, Hideko ; Okano, Teruo ; Lee, Seung‐Jin ; Yamato, Masayuki

Further information

• DOI: 10.1002/mabi.201700073
• URN: urn:nbn:de:101:1-2022100707350393387342
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:23 AM CEST