Molecular identification of diazotroph microbial consortia in mountain soil

Abstract: Nitrogen fixing microbial consortia from soil samples taken from five altitudinal vegetation zones (alpine, subalpine, coniferous, beech, Maleia flood plain) of Parâng Massif, Romania, were isolated and identified. Molecular characterisation of nitrogen fixing consortia was carried out by PCR and nested PCR with 7 primer sets specific to nifH genes. All nifH genes are specific to nitrogen fixation and are found within phylogenetically related organisms which have the nitrogenase enzyme complex. These molecular studies allowed the assessment of nifH gene diversity within these nitrogen fixing microbial consortia from different type of soils. At high altitude, a consortium of nitrogen fixing bacteria dominated by Azotobacter chroococcum and Azospirillum brasilense was found. Clostridium, Rhizobiales, Herbaspirillum, Frankia species were also found in different rations depending on the altitudinal vegetation zone.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Molecular identification of diazotroph microbial consortia in mountain soil ; volume:5 ; number:5 ; year:2010 ; pages:664-673 ; extent:10 ; Open life sciences ; 5, Heft 5 (2010), 664-673 (gesamt 10)

Contributors, Places and Time

• Creator: Carpa, Rahela ; Butiuc-Keul, Anca ; Dobrotă, Cristina ; Muntean, Vasile

Further information

• DOI: 10.2478/s11535-010-0055-8
• URN: urn:nbn:de:101:1-2409201845057.235931226044
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:26 AM CEST