OBE3 and WUS interaction in shoot meristem stem cell regulation
Abstract: The stem cells in the shoot apical meristem (SAM) are the origin of all above ground tissues in plants. In Arabidopsis thaliana, shoot meristem stem cells are maintained by the homeobox transcription factor gene WUS (WUSCHEL) that is expressed in cells of the organizing center underneath the stem cells. In order to identify factors that operate together with WUS in stem cell maintenance, we performed an EMS mutant screen for modifiers of the hypomorphic wus-6 allele.We isolated the oberon3-2 (obe3-2) mutant that enhances stem cell defects in wus-6, but does not affect the putative null allele wus-1. The OBE3 gene encodes a PHD (Plant Homeo Domain) protein that is thought to function in chromatin regulation. Single mutants of OBE3 or its closest homolog OBE4 do not display any defects, whereas the obe3-2 obe4-2 double mutant displays broad growth defects and developmental arrest of seedlings. Transcript levels of WUS and its target gene in the stem cells, CLAVATA3, are reduced in obe3-2. On the other hand, OBE3 and OBE4 transcripts are both indirectly upregulated by ectopic WUS expression. Our results suggest a positive feedback regulation between WUS and OBE3 that contributes to shoot meristem homeostasis
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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PLoS ONE. 11, 5 (2016), e0155657. DOI 10.1371/journal.pone.0155657, issn: 1932-6203
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs
- Keyword
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Meristem
Stammzelle
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2016
- Creator
- Contributor
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Fakultät für Biologie
BIOSS Centre for Biological Signalling Studies
Albert-Ludwigs-Universität Freiburg
- DOI
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10.1371/journal.pone.0155657
- URN
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urn:nbn:de:bsz:25-freidok-122876
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:49 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Lin, Ta-Fang
- Saiga, Shunsuke
- Abe, Mitsutomo
- Laux, Thomas
- Fakultät für Biologie
- BIOSS Centre for Biological Signalling Studies
- Albert-Ludwigs-Universität Freiburg
- Universität
Time of origin
- 2016
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Nitric oxide controls shoot meristem activity via regulation of DNA methylation
Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance
Metabolic regulation of the shoot apical meristem by the TOR kinase in Arabidopsis thaliana
Accession-specific modifiers act with ZWILLE/ARGONAUTE10 to maintain shoot meristem stem cells during embryogenesis in Arabidopsis
ZLL/AGO10 maintains shoot meristem stem cells during Arabidopsis embryogenesis by down-regulating ARF2-mediated auxin response
The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana
A phosphoinositide map at the shoot apical meristem in Arabidopsis thaliana
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Global gene regulatory network underlying miR165a in Arabidopsis shoot apical meristem
A mechanistic framework for non-cell autonomous stem cell induction by WUSCHEL in the Arabidopsis thaliana shoot apical meristem
The Tornado2 gene of Arabidopsis thaliana affectscellular decisions in the shoot apical meristem
Patterning the shoot meristem stem cell niche by miR394 signaling
Nitric oxide controls shoot meristem activity via regulation of DNA methylation
Function of the ARGONAUTE protein ZWILLE in Arabidopsis shoot meristem stem cell maintenance
Metabolic regulation of the shoot apical meristem by the TOR kinase in Arabidopsis thaliana
Accession-specific modifiers act with ZWILLE/ARGONAUTE10 to maintain shoot meristem stem cells during embryogenesis in Arabidopsis
ZLL/AGO10 maintains shoot meristem stem cells during Arabidopsis embryogenesis by down-regulating ARF2-mediated auxin response
The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana
A phosphoinositide map at the shoot apical meristem in Arabidopsis thaliana
Circular RNAs modulate the floral fate acquisition in soybean shoot apical meristem
Global gene regulatory network underlying miR165a in Arabidopsis shoot apical meristem
A mechanistic framework for non-cell autonomous stem cell induction by WUSCHEL in the Arabidopsis thaliana shoot apical meristem