Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation

Abstract: In this study, efficient T cell activation is demonstrated using cell‐sized artificial antigen‐presenting cells (aAPCs) with protein‐conjugated bilayer lipid membranes that mimic biological cell membranes. The highly uniform aAPCs are generated by a facile method based on standard droplet microfluidic devices. These aAPCs are able to activate the T cells in peripheral blood mononuclear cells, showing a 28‐fold increase in interferon gamma (IFNγ) secretion, a 233‐fold increase in antigen‐specific CD8 T cells expansion, and a 16‐fold increase of CD4 T cell expansion. The aAPCs do not require repetitive boosting or additional stimulants and can function at a relatively low aAPC‐to‐T cell ratio (1:17). The research presents strong evidence that the surface fluidity and size of the aAPCs are critical to the effective formation of immune synapses essential for T cell activation. The findings demonstrate that the microfluidic‐generated aAPCs can be instrumental in investigating the physiological conditions and mechanisms for T cell activation. Finally, this method demonstrates the feasibility of customizable aAPCs for a cost‐effective off‐the‐shelf approach to immunotherapy.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Cell‐Sized Lipid Vesicles as Artificial Antigen‐Presenting Cells for Antigen‐Specific T Cell Activation ; day:31 ; month:01 ; year:2023 ; extent:15 ; Advanced healthcare materials ; (31.01.2023) (gesamt 15)

Contributors, Places and Time

• Creator: Chen, Jui‐Yi ; Agrawal, Sudhanshu ; Yi, Hsiu‐Ping ; Vallejo, Derek ; Agrawal, Anshu ; Lee, Abraham P.

Further information

• DOI: 10.1002/adhm.202203163
• URN: urn:nbn:de:101:1-2023020114393065608727
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:23 AM CEST