Neural Tracing Protein‐Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons
Abstract: Neural tracing proteins like horseradish peroxidase‐conjugated wheat germ agglutinin (WGA‐HRP) can target the central nervous system (CNS) through anatomic retrograde transport without crossing the blood–brain barrier (BBB). Conjugating WGA‐HRP to nanoparticles may enable the creation of BBB‐bypassing nanomedicine. Microfluidics and two‐photon confocal microscopy is applied to screen nanocarriers for transport efficacy and gain mechanistic insights into their interactions with neurons. Protein modification of gold nanoparticles alters their cellular uptake at the axonal terminal and activates fast retrograde transport. Trajectory analysis of individual endosomes carrying the nanoparticles reveals a run‐and‐pause pattern along the axon with endosomes carrying WGA‐HRP‐conjugated gold nanoparticles exhibiting longer run duration and faster instantaneous velocity than those carrying nonconjugated nanoparticles. The results offer a mechanistic explanation of the different axonal transport dynamics as well as a cell‐based functional assay of neuron‐targeted nanoparticles with the goal of developing BBB‐bypassing nanomedicine for the treatment of nervous system disorders.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Neural Tracing Protein‐Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons ; day:22 ; month:04 ; year:2024 ; extent:14
Small ; (22.04.2024) (gesamt 14)
- Creator
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Wang, Wenqian
Hassan, Md. Musfizur
Kapoor‐Kaushik, Natasha
Livni, Lital
Musrie, Benjamin
Tang, Jianbo
Mahmud, Zaheri
Lai, Saluo
Wich, Peter Richard
Ananthanarayanan, Vaishnavi
Moalem‐Taylor, Gila
Mao, Guangzhao
- DOI
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10.1002/smll.202311921
- URN
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urn:nbn:de:101:1-2404231405143.654054248585
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:56 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Wang, Wenqian
- Hassan, Md. Musfizur
- Kapoor‐Kaushik, Natasha
- Livni, Lital
- Musrie, Benjamin
- Tang, Jianbo
- Mahmud, Zaheri
- Lai, Saluo
- Wich, Peter Richard
- Ananthanarayanan, Vaishnavi
- Moalem‐Taylor, Gila
- Mao, Guangzhao
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Quantification of retrograde axonal transport in the rat optic nerve by Fluorogold spectrometry
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IGF 1R regulates retrograde axonal transport of signalling endosomes in motor neurons
Analysis of synaptic degeneration : the roles of retrograde axonal transport and homeostatic plasticity
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Quantification of retrograde axonal transport in the rat optic nerve by Fluorogold spectrometry
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Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS
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