Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
Abstract: A bright and photostable fluorescent dye with a disulfide (S−S) linker and maleimide group (Rho594‐S2‐mal), as cleavable and reactive sites, was synthesized and conjugated with anti‐GFP nanobodies (NB). The binding of EGFP (FRET donor) with anti‐GFP NB labeled with one or two Rho594‐S2‐mal residues was studied in vitro and in cellulo. The linker was cleaved with dithiothreitol recovering the donor (FP) signal. The bioconjugates (FP‐NB‐dye) were applied in FRET‐FLIM assays, confocal imaging, and superresolution STED microscopy.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy ; day:16 ; month:08 ; year:2022 ; extent:1
ChemBioChem ; (16.08.2022) (gesamt 1)
- Creator
- DOI
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10.1002/cbic.202200395
- URN
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urn:nbn:de:101:1-2022081715055516531257
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:34 AM CEST
Data provider
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Aktalay, Ayse
- Ponsot, Flavien
- Bossi, Mariano L.
- Belov, Vladimir N.
- Hell, Stefan W.
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