The Length of a Ubiquitin Chain: A General Factor for Selective Recognition by Ubiquitin‐Binding Proteins
Abstract: The attachment of ubiquitin (Ub) chains of various length to proteins is a prevalent posttranslational modification in eukaryotes. The fate of a modified protein is determined by Ub‐binding proteins (UBPs), which interact with Ub chains in a linkage‐selective manner. However, the impact and functional consequences of chain length on the binding selectivity of UBPs remain mostly elusive. We have generated Ub chains of defined length and linkage by using click chemistry and GELFrEE fractionation. These defined polymers were used in affinity‐based enrichment assays to identify length‐ and linkage‐selective interaction partners on a proteome‐wide scale. For the first time, it is revealed that the length of a Ub chain generally has a major impact on its ability to be selectively recognized by UBPs.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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The Length of a Ubiquitin Chain: A General Factor for Selective Recognition by Ubiquitin‐Binding Proteins ; volume:59 ; number:30 ; year:2020 ; pages:12371-12375 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 30 (2020), 12371-12375 (gesamt 5)
- Urheber
- DOI
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10.1002/anie.202003058
- URN
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urn:nbn:de:101:1-2022062006092683349404
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:34 MESZ
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Beteiligte
- Lutz, Joachim
- Höllmüller, Eva
- Scheffner, Martin
- Marx, Andreas
- Stengel, Florian
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