Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast

Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes. In fission yeast, in which the nuclear envelope does not break down during mitosis, Ran targets the centrosomal transforming acidic coiled‐coil (TACC) protein Alp7 for spindle formation. Alp7 accumulates in the nucleus only during mitosis, although its underlying mechanism remains elusive. Here, we investigate the behaviour of Alp7 and its binding partner, Alp14/TOG, throughout the cell cycle. Interestingly, Alp7 enters the nucleus during interphase but is subsequently exported to the cytoplasm by the Exportin‐dependent nuclear export machinery. The continuous nuclear export of Alp7 during interphase is essential for maintaining the array‐like cytoplasmic microtubule structure. The mitosis‐specific nuclear accumulation of Alp7 seems to be under the control of cyclin‐dependent kinase (CDK). These results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC–Alp14/TOG complex through the coordinated interplay of Ran and CDK.
Microtubules undergo drastic spatial reorganization to form bipolar spindles upon mitotic entry. Here, Sato et al show that a conserved complex consisting of Alp7/TACC and Alp14/TOG in fission yeast, which is required for microtubule organization during both interphase and mitosis, shuttles between the cytoplasm and the nucleus,

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast ; volume:10 ; number:10 ; year:2009 ; pages:1161-1167 ; extent:7 ; EMBO reports / European Molecular Biology Organization ; 10, Heft 10 (2009), 1161-1167 (gesamt 7)

Contributors, Places and Time

• Creator: Sato, Masamitsu ; Okada, Naoyuki ; Kakui, Yasutaka ; Yamamoto, Masayuki ; Yoshida, Minoru ; Toda, Takashi

Further information

• DOI: 10.1038/embor.2009.158
• URN: urn:nbn:de:101:1-2023042808325820006329
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:51 AM CEST