Rationalizing the Optimization of Detergents for Membrane Protein Purification
Abstract: Membrane protein purification by means of detergents is key to isolating membrane‐bound therapeutic targets. The role of the detergent structure in this process, however, is not well understood. Detergents are optimized empirically, leading to failed preparations, and thereby raising costs. Here we evaluate the utility of the hydrophilic‐lipophilic balance (HLB) concept, which was introduced by Griffin in 1949, for guiding the optimization of the hydrophobic tail in first‐generation, dendritic oligoglycerol detergents ([G1] OGDs). Our findings deliver qualitative HLB guidelines for rationalizing the optimization of detergents. Moreover, [G1] OGDs exhibit strongly delipidating properties, regardless of the structure of the hydrophobic tail, which delivers a methodological enabling step for investigating binding strengths of endogenous lipids and their role for membrane protein oligomerization. Our findings will facilitate the analysis of challenging drug targets in the future.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Rationalizing the Optimization of Detergents for Membrane Protein Purification ; day:19 ; month:04 ; year:2023 ; extent:14
Chemistry - a European journal ; (19.04.2023) (gesamt 14)
- Urheber
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Urner, Leonhard H.
Junge, Florian
Fiorentino, Francesco
El‐Baba, Tarick J.
Shutin, Denis
Nölte, Gideon
Haag, Rainer
Robinson, Carol V.
- DOI
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10.1002/chem.202300159
- URN
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urn:nbn:de:101:1-2023042415194066825354
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:45 MESZ
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Beteiligte
- Urner, Leonhard H.
- Junge, Florian
- Fiorentino, Francesco
- El‐Baba, Tarick J.
- Shutin, Denis
- Nölte, Gideon
- Haag, Rainer
- Robinson, Carol V.
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