In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii
Abstract: Cyanobacteria are prokaryotic organisms characterized by their ability to produce secondary metabolites with different biological activities. The aim of this work was to evaluate the in vitro and in vivo toxicity of the cosmopolitan freshwater cyanobacterium H. kuetzingii. An extract from H. kuetzingii and cyanobacterial growth media were assessed for presence of intracellular and extracellular toxins by in vitro tests using primary cell cultures from mouse kidney and fibroblasts, cell lines A549 and 3T3, a fish cell line RTgill-W1 as well as by a traditional in vivo mouse bioassay. The presence of toxicity was compared with the ELISA and HPLC data for corresponding cyanotoxins. In vitro tests showed pronounced cytotoxicity of the cyanobacterium extract and growth medium in which H. kuetzingii released potential extracellular toxic compounds as the mammalian cells were significantly more sensitive to exposure compared to the fish cells. Histopathological analyses of the liver and kidneys of treated mice showed pathological changes such as leukocyte infiltration and necrosis, changes in the proximal and distal convoluted tubules, lack of differentiation of Bowman’s space, enlarged Bowman’s capsules and massive hemorrhages. ELISA and HPLC analyses confirmed the presence of saxitoxins and microcystins at low concentrations. In addition, the histological analyses suggest that H. kuetzingii produces other, yet unknown toxic metabolites. Monitoring efforts are therefore required to evaluate the potential hazard for the freshwater aquatic systems and possible public health implications associated with this cyanobacterium.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii ; volume:8 ; number:12 ; year:2013 ; pages:1216-1229 ; extent:14
Open life sciences ; 8, Heft 12 (2013), 1216-1229 (gesamt 14)
- Creator
-
Teneva, Ivanka
Stoyanov, Plamen
Mladenov, Rumen
Dzhambazov, Balik
- DOI
-
10.2478/s11535-013-0239-0
- URN
-
urn:nbn:de:101:1-2409211558512.794958656683
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:29 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Teneva, Ivanka
- Stoyanov, Plamen
- Mladenov, Rumen
- Dzhambazov, Balik
Other Objects (12)
Determination of the toxicity of the freshwater cyanobacterium Woronichinia naegeliana (Unger) Elenkin
Fischerellin, a new allelochemical from the freshwater cyanobacterium Fischerella Muscicola
In vitro and in vivo toxicity assessment of nanoparticles
Highly plastic genome of Microcystis aeruginosa PCC 7806, a ubiquitous toxic freshwater cyanobacterium
Toxicity of single steroid hormones and their mixtures toward the cyanobacterium Microcystis aeruginosa
Assessing the toxicity of gold nanoparticles in vitro and in vivo
In vivo and in vitro toxicity evaluation of liposome-encapsulated sirolimus
Assessing the toxicity of gold nanoparticles in vitro and in vivo
Toxicity of Tire Rubber Microplastics to Freshwater Sediment Organisms
Ingestion and toxicity of polystyrene microplastics in freshwater bivalves
Comparative lung toxicity of engineered nanomaterials utilizing in vitro, ex vivo and in vivo approaches
Oral toxicity of the microcystin-containing cyanobacterium Planktothrix rubescens in European whitefish (Coregonus lavaretus)
Determination of the toxicity of the freshwater cyanobacterium Woronichinia naegeliana (Unger) Elenkin
Fischerellin, a new allelochemical from the freshwater cyanobacterium Fischerella Muscicola
In vitro and in vivo toxicity assessment of nanoparticles
Highly plastic genome of Microcystis aeruginosa PCC 7806, a ubiquitous toxic freshwater cyanobacterium
Toxicity of single steroid hormones and their mixtures toward the cyanobacterium Microcystis aeruginosa
Assessing the toxicity of gold nanoparticles in vitro and in vivo
In vivo and in vitro toxicity evaluation of liposome-encapsulated sirolimus
Assessing the toxicity of gold nanoparticles in vitro and in vivo
Toxicity of Tire Rubber Microplastics to Freshwater Sediment Organisms
Ingestion and toxicity of polystyrene microplastics in freshwater bivalves
Comparative lung toxicity of engineered nanomaterials utilizing in vitro, ex vivo and in vivo approaches
Oral toxicity of the microcystin-containing cyanobacterium Planktothrix rubescens in European whitefish (Coregonus lavaretus)
Determination of the toxicity of the freshwater cyanobacterium Woronichinia naegeliana (Unger) Elenkin
Fischerellin, a new allelochemical from the freshwater cyanobacterium Fischerella Muscicola
In vitro and in vivo toxicity assessment of nanoparticles
Highly plastic genome of Microcystis aeruginosa PCC 7806, a ubiquitous toxic freshwater cyanobacterium
Toxicity of single steroid hormones and their mixtures toward the cyanobacterium Microcystis aeruginosa
Assessing the toxicity of gold nanoparticles in vitro and in vivo
In vivo and in vitro toxicity evaluation of liposome-encapsulated sirolimus
Assessing the toxicity of gold nanoparticles in vitro and in vivo
Toxicity of Tire Rubber Microplastics to Freshwater Sediment Organisms
Ingestion and toxicity of polystyrene microplastics in freshwater bivalves
Comparative lung toxicity of engineered nanomaterials utilizing in vitro, ex vivo and in vivo approaches