Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2

Abstract: Bipolar spindle assembly is necessary to ensure the proper progression of cell division. Loss of spindle pole integrity leads to multipolar spindles and aberrant chromosomal segregation. However, the mechanism underlying the maintenance of spindle pole integrity remains unclear. In this study, we show that the actin‐binding protein adducin‐1 (ADD1) is phosphorylated at S726 during mitosis. S726‐phosphorylated ADD1 localizes to centrosomes, wherein it organizes into a rosette‐like structure at the pericentriolar material. ADD1 depletion causes centriole splitting and therefore results in multipolar spindles during mitosis, which can be restored by re‐expression of ADD1 and the phosphomimetic S726D mutant but not by the S726A mutant. Moreover, the phosphorylation of ADD1 at S726 is crucial for its interaction with TPX2, which is essential for spindle pole integrity. Together, our findings unveil a novel function of ADD1 in maintaining spindle pole integrity through its interaction with TPX2.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 ; volume:19 ; number:8 ; year:2018 ; extent:15 ; EMBO reports / European Molecular Biology Organization ; 19, Heft 8 (2018) (gesamt 15)

Contributors, Places and Time

• Creator: Hsu, Wen‐Hsin ; Wang, Won‐Jing ; Lin, Wan‐Yi ; Huang, Yu‐Min ; Lai, Chien‐Chen ; Liao, Jung‐Chi ; Chen, Hong‐Chen

Further information

• DOI: 10.15252/embr.201745607
• URN: urn:nbn:de:101:1-2022090508014910219431
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:35 AM CEST