Agl24 is an ancient archaeal homolog of the eukaryotic N-glycan chitobiose synthesis enzymes
Abstract: Protein N-glycosylation is a post-translational modification found in organisms of all domains of life. The crenarchaeal N-glycosylation begins with the synthesis of a lipid-linked chitobiose core structure, identical to that in Eukaryotes, although the enzyme catalyzing this reaction remains unknown. Here, we report the identification of a thermostable archaeal β-1,4-N-acetylglucosaminyltransferase, named archaeal glycosylation enzyme 24 (Agl24), responsible for the synthesis of the N-glycan chitobiose core. Biochemical characterization confirmed its function as an inverting β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol glycosyltransferase. Substitution of a conserved histidine residue, found also in the eukaryotic and bacterial homologs, demonstrated its functional importance for Agl24. Furthermore, bioinformatics and structural modeling revealed similarities of Agl24 to the eukaryotic Alg14/13 and a distant relation to the bacterial MurG, which are catalyzing the same or a similar reaction, respectively. Phylogenetic analysis of Alg14/13 homologs indicates that they are ancient in Eukaryotes, either as a lateral transfer or inherited through eukaryogenesis
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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eLife. - 11 (2022) , e67448, ISSN: 2050-084X
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2024
- Creator
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Meyer, Benjamin
Adam, Panagiotis
Wagstaff, Ben A.
Kolyfetis, George E.
Probst, Alexander J.
Albers, Sonja Verena
Dorfmueller, Helge C.
- DOI
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10.7554/elife.67448
- URN
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urn:nbn:de:bsz:25-freidok-2448396
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:46 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Meyer, Benjamin
- Adam, Panagiotis
- Wagstaff, Ben A.
- Kolyfetis, George E.
- Probst, Alexander J.
- Albers, Sonja Verena
- Dorfmueller, Helge C.
- Universität
Time of origin
- 2024
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