Profiling Dynamic Patterns of Single‐Cell Motility
Abstract: Cell motility plays an essential role in many biological processes as cells move and interact within their local microenvironments. Current methods for quantifying cell motility typically involve tracking individual cells over time, but the results are often presented as averaged values across cell populations. While informative, these ensemble approaches have limitations in assessing cellular heterogeneity and identifying generalizable patterns of single‐cell behaviors, at baseline and in response to perturbations. In this study, CaMI is introduced, a computational framework designed to leverage the single‐cell nature of motility data. CaMI identifies and classifies distinct spatio‐temporal behaviors of individual cells, enabling robust classification of single‐cell motility patterns in a large dataset (n = 74 253 cells). This framework allows quantification of spatial and temporal heterogeneities, determination of single‐cell motility behaviors across various biological conditions and provides a visualization scheme for direct interpretation of dynamic cell behaviors. Importantly, CaMI reveals insights that conventional cell motility analyses may overlook, showcasing its utility in uncovering robust biological insights. Together, a multivariate framework is presented to classify emergent patterns of single‐cell motility, emphasizing the critical role of cellular heterogeneity in shaping cell behaviors across populations.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Profiling Dynamic Patterns of Single‐Cell Motility ; day:13 ; month:08 ; year:2024 ; extent:16
Advanced science ; (13.08.2024) (gesamt 16)
- Creator
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Maity, Debonil
Sivakumar, Nikita
Kamat, Pratik
Zamponi, Nahuel
Min, Chanhong
Du, Wenxuan
Jayatilaka, Hasini
Johnston, Adrian
Starich, Bartholomew
Agrawal, Anshika
Riley, Deanna
Venturutti, Leandro
Melnick, Ari
Cerchietti, Leandro
Walston, Jeremy
Phillip, Jude M.
- DOI
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10.1002/advs.202400918
- URN
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urn:nbn:de:101:1-2408131424397.049575014908
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:58 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Maity, Debonil
- Sivakumar, Nikita
- Kamat, Pratik
- Zamponi, Nahuel
- Min, Chanhong
- Du, Wenxuan
- Jayatilaka, Hasini
- Johnston, Adrian
- Starich, Bartholomew
- Agrawal, Anshika
- Riley, Deanna
- Venturutti, Leandro
- Melnick, Ari
- Cerchietti, Leandro
- Walston, Jeremy
- Phillip, Jude M.
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