A global data-driven census of Salmonella small proteins and their potential functions in bacterial virulence
Abstract: Small proteins are an emerging class of gene products with diverse roles in bacterial physiology. However, a full understanding of their importance has been hampered by insufficient genome annotations and a lack of comprehensive characterization in microbes other than Escherichia coli. We have taken an integrative approach to accelerate the discovery of small proteins and their putative virulence-associated functions in Salmonella Typhimurium. We merged the annotated small proteome of Salmonella with new small proteins predicted with in silico and experimental approaches. We then exploited existing and newly generated global datasets that provide information on small open reading frame expression during infection of epithelial cells (dual RNA-seq), contribution to bacterial fitness inside macrophages (Transposon-directed insertion sequencing), and potential engagement in molecular interactions (Grad-seq). This integrative approach suggested a new role for the small protein MgrB beyond its known function in regulating PhoQ. We demonstrate a virulence and motility defect of a Salmonella ΔmgrB mutant and reveal an effect of MgrB in regulating the Salmonella transcriptome and proteome under infection-relevant conditions. Our study highlights the power of interpreting available ‘omics’ datasets with a focus on small proteins, and may serve as a blueprint for a data integration-based survey of small proteins in diverse bacteria
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
microLife. - 1, 1 (2020) , uqaa002, ISSN: 2633-6693
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2024
- Urheber
-
Venturini, Elisa
Svensson, Sarah L.
Maaß, Sandra
Gelhausen, Rick
Eggenhofer, Florian
Li, Lei
Cain, Amy K
Parkhill, Julian
Becher, Dörte
Backofen, Rolf
Barquist, Lars
Sharma, Cynthia M.
Westermann, Alexander J.
Vogel, Jörg
- DOI
-
10.1093/femsml/uqaa002
- URN
-
urn:nbn:de:bsz:25-freidok-2599015
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:21 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Venturini, Elisa
- Svensson, Sarah L.
- Maaß, Sandra
- Gelhausen, Rick
- Eggenhofer, Florian
- Li, Lei
- Cain, Amy K
- Parkhill, Julian
- Becher, Dörte
- Backofen, Rolf
- Barquist, Lars
- Sharma, Cynthia M.
- Westermann, Alexander J.
- Vogel, Jörg
- Universität
Entstanden
- 2024
Ähnliche Objekte (12)
Expressivity and decidability of first-order languages over feature trees
Expressivity and decidability of first-order languages over feature trees
A complete axiomatization of a theory with feature and arity constraints
Regular path expressions in feature logic
How to win a game with features
A complete and recursive feature theory
StoatyDive: evaluation and classification of peak profiles for sequencing data
Computational analyses of post-transcriptional regulatory mechanisms : = Computergestützte Analysen von post-transkriptionellen regulatorischen Mechanismen
Computational analysis and prediction of RNA-RNA interactions : = Computergestützte Analyse und Vorhersage von RNA-RNA-Interaktionen
Using local RNA secondary structures for computational comparison and clustering of RNA molecules
INFO-RNA—a server for fast inverse RNA folding satisfying sequence constraints
In Silico Prediction of Modular Domain-Peptide Interactions
Expressivity and decidability of first-order languages over feature trees
Expressivity and decidability of first-order languages over feature trees
A complete axiomatization of a theory with feature and arity constraints
Regular path expressions in feature logic
How to win a game with features
A complete and recursive feature theory
StoatyDive: evaluation and classification of peak profiles for sequencing data
Computational analyses of post-transcriptional regulatory mechanisms : = Computergestützte Analysen von post-transkriptionellen regulatorischen Mechanismen
Computational analysis and prediction of RNA-RNA interactions : = Computergestützte Analyse und Vorhersage von RNA-RNA-Interaktionen
Using local RNA secondary structures for computational comparison and clustering of RNA molecules
INFO-RNA—a server for fast inverse RNA folding satisfying sequence constraints
In Silico Prediction of Modular Domain-Peptide Interactions
Expressivity and decidability of first-order languages over feature trees
Expressivity and decidability of first-order languages over feature trees
A complete axiomatization of a theory with feature and arity constraints
Regular path expressions in feature logic
How to win a game with features
A complete and recursive feature theory
StoatyDive: evaluation and classification of peak profiles for sequencing data
Computational analyses of post-transcriptional regulatory mechanisms : = Computergestützte Analysen von post-transkriptionellen regulatorischen Mechanismen
Computational analysis and prediction of RNA-RNA interactions : = Computergestützte Analyse und Vorhersage von RNA-RNA-Interaktionen
Using local RNA secondary structures for computational comparison and clustering of RNA molecules
INFO-RNA—a server for fast inverse RNA folding satisfying sequence constraints