RNA polymerase II CTD is dispensable for transcription and required for termination in human cells

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Abstract: The largest subunit of RNA polymerase (Pol) II harbors an evolutionarily conserved C‐terminal domain (CTD), composed of heptapeptide repeats, central to the transcriptional process. Here, we analyze the transcriptional phenotypes of a CTD‐Δ5 mutant that carries a large CTD truncation in human cells. Our data show that this mutant can transcribe genes in living cells but displays a pervasive phenotype with impaired termination, similar to but more severe than previously characterized mutations of CTD tyrosine residues. The CTD‐Δ5 mutant does not interact with the Mediator and Integrator complexes involved in the activation of transcription and processing of RNAs. Examination of long‐distance interactions and CTCF‐binding patterns in CTD‐Δ5 mutant cells reveals no changes in TAD domains or borders. Our data demonstrate that the CTD is largely dispensable for the act of transcription in living cells. We propose a model in which CTD‐depleted Pol II has a lower entry rate onto DNA but becomes pervasive once engaged in transcription, resulting in a defect in termination.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: RNA polymerase II CTD is dispensable for transcription and required for termination in human cells ; day:10 ; month:07 ; year:2023 ; extent:17
EMBO reports / European Molecular Biology Organization ; (10.07.2023) (gesamt 17)

Creator: Yahia, Yousra
Pigeot, Alexia
El Aabidine, Amal Zine
Shah, Nilay
Karasu, Nezih
Forné, Ignasi
Krebs, Stefan
Blum, Helmut
Esnault, Cyril
Sexton, Tom
Imhof, Axel
Eick, Dirk
Andrau, Jean‐Christophe

DOI: 10.15252/embr.202256150

URN: urn:nbn:de:101:1-2023071015385072568265

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 10:48 AM CEST

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Associated

Yahia, Yousra
Pigeot, Alexia
El Aabidine, Amal Zine
Shah, Nilay
Karasu, Nezih
Forné, Ignasi
Krebs, Stefan
Blum, Helmut
Esnault, Cyril
Sexton, Tom
Imhof, Axel
Eick, Dirk
Andrau, Jean‐Christophe

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Maternal DOT1L is dispensable for mouse development

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FAM111A is dispensable for electrolyte homeostasis in mice

Termination proofs for logic programs

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RNA polymerase II CTD is dispensable for transcription and required for termination in human cells

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Other Objects (12)

Insights into the RNA Polymerase CTD code

Insights into the RNA polymerase II CTD code

Functional analysis of Tyrosine-1 of mammalian RNA polymerase II CTD

Mechanism of polyadenylation-independent RNA polymerase II termination

Termination of non‐coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD‐mimicking region in Sen1

Structural basis of Integrator-dependent RNA polymerase II termination

Maternal DOT1L is dispensable for mouse development

Chchd10 is dispensable for myogenesis but critical for adipose browning

DNAJB7 is dispensable for male fertility in mice

FAM111A is dispensable for electrolyte homeostasis in mice

Termination proofs for logic programs

Termination proofs for logic programs

Insights into the RNA Polymerase CTD code

Insights into the RNA polymerase II CTD code

Functional analysis of Tyrosine-1 of mammalian RNA polymerase II CTD

Mechanism of polyadenylation-independent RNA polymerase II termination

Termination of non‐coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD‐mimicking region in Sen1

Structural basis of Integrator-dependent RNA polymerase II termination

Maternal DOT1L is dispensable for mouse development

Chchd10 is dispensable for myogenesis but critical for adipose browning

DNAJB7 is dispensable for male fertility in mice

FAM111A is dispensable for electrolyte homeostasis in mice

Termination proofs for logic programs

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Termination of non‐coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD‐mimicking region in Sen1

Structural basis of Integrator-dependent RNA polymerase II termination

Maternal DOT1L is dispensable for mouse development

Chchd10 is dispensable for myogenesis but critical for adipose browning

DNAJB7 is dispensable for male fertility in mice

FAM111A is dispensable for electrolyte homeostasis in mice

Termination proofs for logic programs

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