A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells
Abstract: A modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells ; volume:62 ; number:5 ; year:2007 ; pages:529-532 ; extent:4
Biologia ; 62, Heft 5 (2007), 529-532 (gesamt 4)
- Creator
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Sun, Wei
Cao, Ziyi
Li, Yan
Zhao, Yanxiu
Zhang, Hui
- DOI
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10.2478/s11756-007-0104-6
- URN
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urn:nbn:de:101:1-2409221547239.683701170071
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Sun, Wei
- Cao, Ziyi
- Li, Yan
- Zhao, Yanxiu
- Zhang, Hui
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