Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants
Abstract: Many DNA aptamers have been reported to target the spike protein (S‐protein) of SARS‐CoV‐2. These aptamers display different affinities or recognize different epitopes of the S‐protein. We conducted a comparative study of 9 DNA aptamers for binding to several variants of the S‐protein and pseudoviruses using the same testing methods, including dot‐blot assays and enzyme‐linked aptamer binding assays, to evaluate their affinity ranking and analytical utility. Moreover, the binding sites of these aptamers on the S‐protein were examined using aptamer competition assays to understand the effect of S‐protein mutations on aptamer affinity and the degree of overlapping of the binding sites by these aptamers.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS‐CoV‐2 Variants ; day:15 ; month:02 ; year:2023 ; extent:11
Analysis & sensing ; (15.02.2023) (gesamt 11)
- Urheber
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Zhang, Zijie
Li, Jiuxing
Amini, Ryan
Mansfield, Alexandria
Gu, Jimmy
Xia, Jianrun
Brennan, John D.
Li, Yingfu
- DOI
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10.1002/anse.202300001
- URN
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urn:nbn:de:101:1-2023021514304918884786
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 11:05 MESZ
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Beteiligte
- Zhang, Zijie
- Li, Jiuxing
- Amini, Ryan
- Mansfield, Alexandria
- Gu, Jimmy
- Xia, Jianrun
- Brennan, John D.
- Li, Yingfu
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