Inactivation of the RNA helicase CrhR impacts a specific subset of the transcriptome in the cyanobacterium Synechocystis sp. PCC 6803
Abstract: DEAD-box RNA-helicases catalyze the reorganization of structured RNAs and the formation of RNP complexes. The cyanobacterium Synechocystis sp. PCC 6803 encodes a single DEAD-box RNA helicase, CrhR (Slr0083), whose expression is regulated by abiotic stresses that alter the redox potential of the photosynthetic electron transport chain, including temperature downshift. Despite its proposed effect on RNA metabolism and its known relevance in cold-stress adaptation, the reported impact of a CrhR knockout on the cold adaption of the transcriptome only identified eight affected genes. Here, we utilized a custom designed microarray to assess the impact of the absence of CrhR RNA helicase activity on the transcriptome, independent of cold stress. CrhR truncation impacts an RNA subset comprising ~10% of the ncRNA and also ~10% of the mRNA transcripts. While equal numbers of mRNAs showed increased as well as decreased abundance, more than 90% of the ncRNAs showed enhanced expression in the absence of CrhR, indicative of a negative effect on ncRNA transcription or stability. We further tested the effect of CrhR on the stability of strongly responding RNAs that identify examples of post-transcriptional and transcriptional regulation. The data suggest that CrhR impacts multiple aspects of RNA metabolism in Synechocystis
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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RNA biology. - 16, 9 (2019) , 1205-1214, ISSN: 1547-6286
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2020
- Creator
- Contributor
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FRIAS Natur- und Lebenswissenschaften, Medizin und Ingenieurwissenschaften
- DOI
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10.1080/15476286.2019.1621622
- URN
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urn:nbn:de:bsz:25-freidok-1533995
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:46 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Georg, Jens
- Rosana, Albert Remus R.
- Chamot, Danuta
- Migur, Anzhela
- Hess, Wolfgang
- Owttrim, George W.
- FRIAS Natur- und Lebenswissenschaften, Medizin und Ingenieurwissenschaften
- Universität
Time of origin
- 2020
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Ecophysiology of the marine cyanobacterium Trichodesmium
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Expression of the plant Photosystem II core proteins in the cyanobacterium Synechocystis sp. PCC6803 and characterization of the DEAD-box RNA helicase RH50 of A. thaliana
Dissecting the role of CRH and CRH-R1 in Alzheimer´s disease
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Insights into the activation of the spliceosomal helicase Prp43
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The subset sum game revisited
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Ecophysiology of the marine cyanobacterium Trichodesmium
Ecophysiology of the marine cyanobacterium Trichodesmium
Expression of the plant Photosystem II core proteins in the cyanobacterium Synechocystis sp. PCC6803 and characterization of the DEAD-box RNA helicase RH50 of A. thaliana
Dissecting the role of CRH and CRH-R1 in Alzheimer´s disease
Dissecting the role of CRH and CRH-R1 in Alzheimer´s disease
Insights into the activation of the spliceosomal helicase Prp43
A type III-Dv CRISPR-Cas system is controlled by the transcription factor RpaB and interacts with the DEAD-box RNA helicase CrhR
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