A Highly Sensitive CRISPR‐Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar‐Level Real‐Time Quantification
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids. Herein, the development of a surface plasmon resonance (SPR) sensor that is functionalized with a layer of locally grown graphdiyne film, achieving excellent sensing performance when coupled with catalytically deactivated CRISPR‐associated protein 9 (dCas9), is reported. dCas9 protein is immobilized on the sensor surface and complexed with a specific single‐guide RNA, enabling the amplification‐free detection of target sequences within genomic DNA. The sensor, termed CRISPR‐SPR‐Chip, is used to successfully analyze recombinant plasmids with only three‐base mutations with a limit of detection as low as 1.3 fM. Real‐time monitoring CRISPR‐SPR‐Chip is used to analyze clinical samples of patients with Duchenne muscular dystrophy with two exon deletions, which are detected without any pre‐amplification step, yielding significantly positive results within 5 min. The ability of this novel CRISPR‐empowered SPR (CRISPR‐eSPR) sensing platform to rapidly, precisely, sensitively, and specifically detect a target gene sequence provides a new on‐chip optic approach for clinical gene analysis.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
A Highly Sensitive CRISPR‐Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar‐Level Real‐Time Quantification ; day:27 ; month:03 ; year:2022 ; extent:14
Advanced science ; (27.03.2022) (gesamt 14)
- Creator
-
Zheng, Fei
Chen, Zhi
Li, Jingfeng
Wu, Rui
Zhang, Bin
Nie, Guohui
Xie, Zhongjian
Zhang, Han
- DOI
-
10.1002/advs.202105231
- URN
-
urn:nbn:de:101:1-2022032815033873655576
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:38 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zheng, Fei
- Chen, Zhi
- Li, Jingfeng
- Wu, Rui
- Zhang, Bin
- Nie, Guohui
- Xie, Zhongjian
- Zhang, Han
Other Objects (12)
Analysis of bridge vibration response for identification of bridge damage using BP neural network
Shearigami: Self‐Folding via Shear Deformation
Hepatitis C Virus (HCV) Specific Sequences Are Demonstrable in the DNA Fraction of Peripheral Blood Mononuclear Cells from Healthy, Anti-HCV Antibody-Negative Individuals and Cell Lines of Human Origin
Removal of arsenic from drinking water using rice husk
Sign-changing multi-bump solutions for the Chern-Simons-Schrödinger equations in ℝ 2
Mixed synthesis method of motion and path of planar four-bar linkages
Dirhodium‐Catalyzed Enantioselective Synthesis of Difluoromethylated Cyclopropanes via Enyne Cycloisomerization
Tim-3 expression and its role in hepatocellular carcinoma
Effects of climate and forest age on the ecosystem carbon exchange of afforestation
Application of Digital PCR in Detecting Human Diseases Associated Gene Mutation
The putative mechanistic insights on how SARS-CoV-2 might influence the outcomes in cancer patients
Source-to-Sink Pathways of Dissolved Organic Carbon in the River-Estuary-Ocean Continuum: A Modeling Investigation
Analysis of bridge vibration response for identification of bridge damage using BP neural network
Shearigami: Self‐Folding via Shear Deformation
Hepatitis C Virus (HCV) Specific Sequences Are Demonstrable in the DNA Fraction of Peripheral Blood Mononuclear Cells from Healthy, Anti-HCV Antibody-Negative Individuals and Cell Lines of Human Origin
Removal of arsenic from drinking water using rice husk
Sign-changing multi-bump solutions for the Chern-Simons-Schrödinger equations in ℝ 2
Mixed synthesis method of motion and path of planar four-bar linkages
Dirhodium‐Catalyzed Enantioselective Synthesis of Difluoromethylated Cyclopropanes via Enyne Cycloisomerization
Tim-3 expression and its role in hepatocellular carcinoma
Effects of climate and forest age on the ecosystem carbon exchange of afforestation
Application of Digital PCR in Detecting Human Diseases Associated Gene Mutation
The putative mechanistic insights on how SARS-CoV-2 might influence the outcomes in cancer patients
Source-to-Sink Pathways of Dissolved Organic Carbon in the River-Estuary-Ocean Continuum: A Modeling Investigation
Analysis of bridge vibration response for identification of bridge damage using BP neural network
Shearigami: Self‐Folding via Shear Deformation
Hepatitis C Virus (HCV) Specific Sequences Are Demonstrable in the DNA Fraction of Peripheral Blood Mononuclear Cells from Healthy, Anti-HCV Antibody-Negative Individuals and Cell Lines of Human Origin
Removal of arsenic from drinking water using rice husk
Sign-changing multi-bump solutions for the Chern-Simons-Schrödinger equations in ℝ 2
Mixed synthesis method of motion and path of planar four-bar linkages
Dirhodium‐Catalyzed Enantioselective Synthesis of Difluoromethylated Cyclopropanes via Enyne Cycloisomerization
Tim-3 expression and its role in hepatocellular carcinoma
Effects of climate and forest age on the ecosystem carbon exchange of afforestation
Application of Digital PCR in Detecting Human Diseases Associated Gene Mutation
The putative mechanistic insights on how SARS-CoV-2 might influence the outcomes in cancer patients