Integrin‐Targeting Fluorescent Proteins: Exploration of RGD Insertion Sites
Abstract: The potential of the fluorescent protein scaffold to control peptide sequence functionality is illustrated by an exploration of fluorescent proteins as novel probes for targeting integrins. A library of fluorescent mCitrine proteins with RGD motifs incorporated at several positions in loops within the protein main chain was generated and characterized. Amino acid mutations to RGD as well as RGD insertions were evaluated: both led to constructs with typical mCitrine fluorescent properties. Screening experiments against four human integrin receptors revealed two strong‐binding constructs and two selective integrin binders. The effect of the site of RGD incorporation illustrates the importance of the protein scaffold on RGD sequence functionality, leading to fluorescent protein constructs with the potential for selective integrin targeting.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Integrin‐Targeting Fluorescent Proteins: Exploration of RGD Insertion Sites ; volume:18 ; number:5 ; year:2017 ; pages:441-443 ; extent:3
ChemBioChem ; 18, Heft 5 (2017), 441-443 (gesamt 3)
- Urheber
- DOI
-
10.1002/cbic.201600514
- URN
-
urn:nbn:de:101:1-2022092305430317119238
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:30 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Sonntag, Michael H.
- Schill, Jurgen
- Brunsveld, Luc
Ähnliche Objekte (12)
Phenothiazine‐Biaryl‐Containing Fluorescent RGD Peptides
Focal adhesion stabilization by enhanced integrin- c RGD binding affinity
Bicyclic RGD peptides : Novel high-affinity ligands for selective integrin-binding and integrin-mediated cell adhesion
How different albumin-binders drive probe distribution of fluorescent RGD mimetics
Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation
Interface Immobilization Chemistry of c RGD‐based Peptides Regulates Integrin Mediated Cell Adhesion
RGD Peptidomimetic MMAE‐Conjugate Addressing Integrin αVβ3‐Expressing Cells with High Targeting Index **
![Einfluß αvβ3-Integrin [Alpha-v-Beta-3-Integrin] spezifisch inhibierenden RGD-Peptide auf den Verlauf einer mesangioproliferativen Glomerulonephritis in der Maus](/assets/placeholder/searchResultMediaNoDigitisedMedia.png)
Einfluß αvβ3-Integrin [Alpha-v-Beta-3-Integrin] spezifisch inhibierenden RGD-Peptide auf den Verlauf einer mesangioproliferativen Glomerulonephritis in der Maus
RGD-decorated conjugated polymer particles as fluorescent biomedical probes prepared by Sonogashira dispersion polymerization
Der Einfluß cyclischer RGD-Peptide auf die Wechselwirkung Fibronectin-Integrin α5β1[alpha 5 beta 1]
Beschichtung künstlicher Oberflächen mit hochaktiven und für das αvβ3-Integrin [Alpha-v-Beta-Integrin] selektiven RGD-Peptiden zur Stimulation der integrinvermittelten Zelladhäsion
Recombinant RGD-disintegrin DisBa-01 blocks integrin αvβ3 and impairs VEGF signaling in endothelial cells
Phenothiazine‐Biaryl‐Containing Fluorescent RGD Peptides
Focal adhesion stabilization by enhanced integrin- c RGD binding affinity
Bicyclic RGD peptides : Novel high-affinity ligands for selective integrin-binding and integrin-mediated cell adhesion
How different albumin-binders drive probe distribution of fluorescent RGD mimetics
Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation
Interface Immobilization Chemistry of c RGD‐based Peptides Regulates Integrin Mediated Cell Adhesion
RGD Peptidomimetic MMAE‐Conjugate Addressing Integrin αVβ3‐Expressing Cells with High Targeting Index **
Einfluß αvβ3-Integrin [Alpha-v-Beta-3-Integrin] spezifisch inhibierenden RGD-Peptide auf den Verlauf einer mesangioproliferativen Glomerulonephritis in der Maus
RGD-decorated conjugated polymer particles as fluorescent biomedical probes prepared by Sonogashira dispersion polymerization
Der Einfluß cyclischer RGD-Peptide auf die Wechselwirkung Fibronectin-Integrin α5β1[alpha 5 beta 1]
Beschichtung künstlicher Oberflächen mit hochaktiven und für das αvβ3-Integrin [Alpha-v-Beta-Integrin] selektiven RGD-Peptiden zur Stimulation der integrinvermittelten Zelladhäsion
Recombinant RGD-disintegrin DisBa-01 blocks integrin αvβ3 and impairs VEGF signaling in endothelial cells
Phenothiazine‐Biaryl‐Containing Fluorescent RGD Peptides
Focal adhesion stabilization by enhanced integrin- c RGD binding affinity
Bicyclic RGD peptides : Novel high-affinity ligands for selective integrin-binding and integrin-mediated cell adhesion
How different albumin-binders drive probe distribution of fluorescent RGD mimetics
Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation
Interface Immobilization Chemistry of c RGD‐based Peptides Regulates Integrin Mediated Cell Adhesion
RGD Peptidomimetic MMAE‐Conjugate Addressing Integrin αVβ3‐Expressing Cells with High Targeting Index **
Einfluß αvβ3-Integrin [Alpha-v-Beta-3-Integrin] spezifisch inhibierenden RGD-Peptide auf den Verlauf einer mesangioproliferativen Glomerulonephritis in der Maus
RGD-decorated conjugated polymer particles as fluorescent biomedical probes prepared by Sonogashira dispersion polymerization
Der Einfluß cyclischer RGD-Peptide auf die Wechselwirkung Fibronectin-Integrin α5β1[alpha 5 beta 1]
Beschichtung künstlicher Oberflächen mit hochaktiven und für das αvβ3-Integrin [Alpha-v-Beta-Integrin] selektiven RGD-Peptiden zur Stimulation der integrinvermittelten Zelladhäsion