Downregulation of circ_0037655 impedes glioma formation and metastasis via the regulation of miR-1229-3p/ITGB8 axis
Background: Glioma is the most frequent, highly aggressive primary intracranial malignant tumor. Circular RNA (circRNA) circ_0037655 has been reported to be a vital regulator in glioma. The different functional mechanism behind circ_0037655 was investigated in the current study. Methods: The expression of circ_0037655, microRNA-1229-3p (miR-1229-3p) and integrin beta-8 (ITGB8) was detected via the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular research was performed via colony formation assay for cell proliferation, flow cytometry for cell cycle and cell apoptosis, scratch assay for cell migration, as well as transwell assay for cell migration and invasion. Western blot was used for detection of ITGB8 protein and epithelial–mesenchymal transition (EMT) process. Dual-luciferase reporter assay was implemented for the binding analysis of potential targets. In vivo assay was administered via xenograft in mice. Results: Upregulation of circ_0037655 was affirmed in glioma samples and cells. Tumor formation and metastasis of glioma were inhibited after circ_0037655 was downregulated. miR-1229-3p acted as a target of circ_0037655, and its upregulation was responsible for the function of si-circ_0037655 in glioma cells. miR-1229-3p functioned as a tumor inhibitor in glioma progression by targeting ITGB8. circ_0037655 modulated the ITGB8 expression by targeting miR-1229-3p. In vivo knockdown of circ_0037655 also suppressed glioma tumorigenesis by acting on the miR-1229-3p/ITGB8 axis. Conclusion: This study showed that downregulation of the expression of circ_0037655 could inhibit glioma progression by acting on the miR-1229-3p/ITGB8 axis. The specific circ_0037655/miR-1229-3p/ITGB8 axis was disclosed in glioma research.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Downregulation of circ_0037655 impedes glioma formation and metastasis via the regulation of miR-1229-3p/ITGB8 axis ; volume:16 ; number:1 ; year:2021 ; pages:442-454 ; extent:13
Open life sciences ; 16, Heft 1 (2021), 442-454 (gesamt 13)
- Creator
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Zou, Wenhui
Cao, Yalei
Cheng, Kai
Li, Changyu
Zhu, Fu
Yang, Shumao
Jin, Maolin
Song, Shaojun
- DOI
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10.1515/biol-2021-0048
- URN
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urn:nbn:de:101:1-2022091914591267011401
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:31 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zou, Wenhui
- Cao, Yalei
- Cheng, Kai
- Li, Changyu
- Zhu, Fu
- Yang, Shumao
- Jin, Maolin
- Song, Shaojun
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circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation
Downregulation of circ-YES1 suppresses NSCLC migration and proliferation through the miR-142-3p–HMGB1 axis
Hsa_circ_0004712 downregulation attenuates ovarian cancer malignant development by targeting the miR-331-3p/FZD4 pathway
Correction to: Downregulation of circ-YES1 suppresses NSCLC migration and proliferation through the miR-142-3p–HMGB1 axis
Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p
Down-regulation of circ_0058058 suppresses proliferation, angiogenesis and metastasis in multiple myeloma through miR-338-3p/ATG14 pathway
Downregulation of circ-Foxo3 in breast cancer stem-like cells
Circ_ZNF124 promotes non-small cell lung cancer progression by abolishing miR-337-3p mediated downregulation of JAK2/STAT3 signaling pathway
CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
Circ_0083964 knockdown impedes rheumatoid arthritis progression via the miR-204-5p-dependent regulation of YY1
miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1
circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis
circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation
Downregulation of circ-YES1 suppresses NSCLC migration and proliferation through the miR-142-3p–HMGB1 axis
Hsa_circ_0004712 downregulation attenuates ovarian cancer malignant development by targeting the miR-331-3p/FZD4 pathway
Correction to: Downregulation of circ-YES1 suppresses NSCLC migration and proliferation through the miR-142-3p–HMGB1 axis
Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p
Down-regulation of circ_0058058 suppresses proliferation, angiogenesis and metastasis in multiple myeloma through miR-338-3p/ATG14 pathway
Downregulation of circ-Foxo3 in breast cancer stem-like cells
Circ_ZNF124 promotes non-small cell lung cancer progression by abolishing miR-337-3p mediated downregulation of JAK2/STAT3 signaling pathway
CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
Circ_0083964 knockdown impedes rheumatoid arthritis progression via the miR-204-5p-dependent regulation of YY1
miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1