Production of isomelezitose from sucrose by engineered glucansucrases

Abstract: Certain lactic acid bacteria produce glycosyltransferases known as glucansucrases, which synthesize α-D-glucans via glucosyl transfer from sucrose. We recently reported on the formation of the unusual trisaccharide isomelezitose in low yields by a variety of glucansucrases. Isomelezitose is a rare non-reducing trisaccharide, with the structure α-d-glucopyranosyl- (1→6)-β-d-fructofuranosyl-(2↔1)-α-d-glucopyranoside. In this work, we describe the synthesis of isomelezitose in high yields by variants of glucansucrases engineered to contain a single point mutation at a key leucine residue involved in acceptor substrate binding. Some variants produce isomelezitose in yields up to 57%. This method is amenable to large-scale production of isomelezitose for food, industrial and biomedical applications.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Production of isomelezitose from sucrose by engineered glucansucrases ; volume:1 ; number:1 ; year:2017 ; pages:82-93 ; extent:12 ; Amylase ; 1, Heft 1 (2017), 82-93 (gesamt 12)

Contributors, Places and Time

• Creator: Côté, Gregory L. ; Dunlap, Christopher A. ; Vermillion, Karl E. ; Skory, Christopher D.

Further information

• DOI: 10.1515/amylase-2017-0008
• URN: urn:nbn:de:101:1-2404291539287.258523812979
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:48 AM CEST