Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
Abstract: Single‐molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to investigate the dynamics of biomolecular events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the molecules. Herein, we introduce a method for kinetic analysis based on control of fluorescence blinking (KACB), a general approach to investigate the dynamics of biomolecules by using a single fluorophore. By controlling the kinetics of the redox reaction the blinking kinetics or pattern can be controlled to be affected by microenvironmental changes around a fluorophore (rKACB), thereby enabling real‐time single‐molecule measurement of the structure‐changing dynamics of nucleic acids.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking ; volume:56 ; number:48 ; year:2017 ; pages:15329-15333 ; extent:5
Angewandte Chemie / International edition. International edition ; 56, Heft 48 (2017), 15329-15333 (gesamt 5)
- Creator
- DOI
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10.1002/anie.201708705
- URN
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urn:nbn:de:101:1-2022091406050512890216
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:36 AM CEST
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kawai, Kiyohiko
- Miyata, Takafumi
- Shimada, Naohiko
- Ito, Syoji
- Miyasaka, Hiroshi
- Maruyama, Atsushi
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Single-molecule fluorescence excitation spectroscopy
Light-Induced Switching of Tunable Single-Molecule Junctions
Light‐Induced Switching of Tunable Single‐Molecule Junctions
Single-Molecule Ultrafast Fluorescence-Detected Pump–Probe Microscopy
Advancing quantitative DNA-mediated single-molecule fluorescence microscopy
Fluorescence depletion by stimulated emission in single-molecule spectroscopy
Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
Controllable Spin Switching in a Single-Molecule Magnetic Tunneling Junction
Single-molecule fluorescence with a spatial resolution smaller 10 nanometer
Single-molecule fluorescence studies of protein folding and molecular chaperones
Polyproline and the "spectroscopic ruler" revisited with single-molecule fluorescence
Single‐Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
Single-molecule fluorescence excitation spectroscopy
Light-Induced Switching of Tunable Single-Molecule Junctions
Light‐Induced Switching of Tunable Single‐Molecule Junctions
Single-Molecule Ultrafast Fluorescence-Detected Pump–Probe Microscopy
Advancing quantitative DNA-mediated single-molecule fluorescence microscopy
Fluorescence depletion by stimulated emission in single-molecule spectroscopy
Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
Controllable Spin Switching in a Single-Molecule Magnetic Tunneling Junction
Single-molecule fluorescence with a spatial resolution smaller 10 nanometer
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