Spindle motility skews division site determination during asymmetric cell division in Physcomitrella
Abstract: Asymmetric cell division (ACD) underlies the development of multicellular organisms. In animal ACD, the cell division site is determined by active spindle-positioning mechanisms. In contrast, it is considered that the division site in plants is determined prior to mitosis by the microtubule-actin belt known as the preprophase band (PPB) and that the localization of the mitotic spindle is typically static and does not govern the division plane. However, in some plant species, ACD occurs in the absence of PPB. Here, we isolate a hypomorphic mutant of the conserved microtubule-associated protein TPX2 in the moss Physcomitrium patens (Physcomitrella) and observe spindle motility during PPB-independent cell division. This defect compromises the position of the division site and produces inverted daughter cell sizes in the first ACD of gametophore (leafy shoot) development. The phenotype is rescued by restoring endogenous TPX2 function and, unexpectedly, by depolymerizing actin filaments. Thus, we identify an active spindle-positioning mechanism that, reminiscent of acentrosomal ACD in animals, involves microtubules and actin filaments, and sets the division site in plants
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Nature communications. - 13, 1 (2022) , 2488, ISSN: 2041-1723
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2022
- Creator
- DOI
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10.1038/s41467-022-30239-1
- URN
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urn:nbn:de:bsz:25-freidok-2284640
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kozgunova, Elena
- Yoshida, Mari W.
- Reski, Ralf
- Goshima, Gohta
- Universität
Time of origin
- 2022
Other Objects (12)
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Zell- und molekularbiologische Untersuchungen der Cytokinin-induzierbaren Gewebedifferenzierung und Chloroplastenteilung bei Physcomitrella patens (Hedw.) B.S.G.
Microscopy of Physcomitrella patens sperm cells
Quantitative Analyse des auxininduzierten miR160/ARF-Regelkreises in Physcomitrella patens
The homeobox gene BELL1 is the master regulator for the developmental switch from gametophyte to sporophyte in Physcomitrella patens
Protein encoding genes in an ancient plant: analysis of codon usage, retained genes and splice sites in a moss, Physcomitrella patens
The moss bioreactor offers best of both worlds for biopharmaceutical production
Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: a review
Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella
Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells
Cytological analysis and structural quantification of FtsZ1-2 and FtsZ2-1 network characteristics in Physcomitrella patens
Zell- und molekularbiologische Untersuchungen der Cytokinin-induzierbaren Gewebedifferenzierung und Chloroplastenteilung bei Physcomitrella patens (Hedw.) B.S.G.
Microscopy of Physcomitrella patens sperm cells
Quantitative Analyse des auxininduzierten miR160/ARF-Regelkreises in Physcomitrella patens
The homeobox gene BELL1 is the master regulator for the developmental switch from gametophyte to sporophyte in Physcomitrella patens
Protein encoding genes in an ancient plant: analysis of codon usage, retained genes and splice sites in a moss, Physcomitrella patens
The moss bioreactor offers best of both worlds for biopharmaceutical production
Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: a review
Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella
Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells