Single‐Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide

Abstract: The first single‐molecule fluorescence detection of a structurally‐defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface‐tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single‐molecule level. The diverse and dynamic nature of HS–protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof‐of‐principle demonstration of the feasibility of single‐molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
Single‐Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide ; volume:17 ; number:21 ; year:2016 ; pages:3442-3446 ; extent:5
ChemPhysChem ; 17, Heft 21 (2016), 3442-3446 (gesamt 5)

Creator
Dalton, Charlotte E.
Quinn, Steven D.
Rafferty, Aidan
Morten, Michael J.
Gardiner, John M.
Magennis, Steven W.

DOI
10.1002/cphc.201600750
URN
urn:nbn:de:101:1-2022110804305741483640
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:30 AM CEST

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Associated

  • Dalton, Charlotte E.
  • Quinn, Steven D.
  • Rafferty, Aidan
  • Morten, Michael J.
  • Gardiner, John M.
  • Magennis, Steven W.

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