Overexpression of phytochelatin synthase (AtPCS) in rice for tolerance to cadmium stress
Abstract: Phytoremediation is an important strategy adapted by plants to sequester and/or detoxify pollutants. Phytochelatins, a family of cysteine-rich thiol-reactive peptides, bind to various heavy metals and metalloids making them good candidates for phytoremediation. Phytochelatin synthase catalyses the final step in the biosynthesis of phytochelatins and can be used as a strategy to improve tolerance against heavy metals. In the present study, an AtPCS gene was overexpressed in rice following the in planta transformation approach. Stringent screening strategies were standardized to select putative transformants under a Cd stress of 125 μM at both seedling and plant levels. Molecular analysis by PCR in 18 tolerant plants confirmed the transgene integration and absence of Agrobacterium. Genomic Southern analysis further confirmed the integration of the T-DNA as a single copy. The stability of the T-DNA in the progeny of 5 selected T1 generation plants was confirmed by tolerance assay, molecular characterization and biochemical analysis for the reduced glutathione, phytochelatin content and lipid peroxidation. This strategy is discussed as a potential mechanism to enhance the tolerance of rice plants to Cd stress.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Overexpression of phytochelatin synthase (AtPCS) in rice for tolerance to cadmium stress ; volume:66 ; number:6 ; year:2011 ; pages:1060-1073 ; extent:14
Biologia ; 66, Heft 6 (2011), 1060-1073 (gesamt 14)
- Urheber
-
Venkataramaiah, Nagaveni
Vudayagiri Ramakrishna, Sashidhar
Sreevathsa, Rohini
- DOI
-
10.2478/s11756-011-0135-x
- URN
-
urn:nbn:de:101:1-2409221717582.784951047013
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:21 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Venkataramaiah, Nagaveni
- Vudayagiri Ramakrishna, Sashidhar
- Sreevathsa, Rohini
Ähnliche Objekte (12)
Overexpression of OsDUF6 increases salt stress tolerance in rice
Comparative study of four rice cultivars with different levels of cadmium tolerance
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of OsHAD3, a Member of HAD Superfamily, Decreases Drought Tolerance of Rice
Enhanced saccharification of rice straw by overexpression of rice exo-glucanase
Overexpression of OsLCT2, a Low-Affinity Cation Transporter Gene, Reduces Cadmium Accumulation in Shoots and Grains of Rice
A cadmium stress-responsive gene AtFC1 confers plant tolerance to cadmium toxicity
Genetic loci regulating cadmium content in rice grains
Proteomic Analysis of Rice Subjected to Low Light Stress and Overexpression of OsGAPB Increases the Stress Tolerance
Clinical significance of Phosphatidyl Inositol Synthase overexpression in oral cancer
Ceramide synthase 4 overexpression exerts oncogenic properties in breast cancer
Overexpression of OsDUF6 increases salt stress tolerance in rice
Comparative study of four rice cultivars with different levels of cadmium tolerance
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of OsHAD3, a Member of HAD Superfamily, Decreases Drought Tolerance of Rice
Enhanced saccharification of rice straw by overexpression of rice exo-glucanase
Overexpression of OsLCT2, a Low-Affinity Cation Transporter Gene, Reduces Cadmium Accumulation in Shoots and Grains of Rice
A cadmium stress-responsive gene AtFC1 confers plant tolerance to cadmium toxicity
Genetic loci regulating cadmium content in rice grains
Proteomic Analysis of Rice Subjected to Low Light Stress and Overexpression of OsGAPB Increases the Stress Tolerance
Clinical significance of Phosphatidyl Inositol Synthase overexpression in oral cancer
Ceramide synthase 4 overexpression exerts oncogenic properties in breast cancer
Overexpression of OsDUF6 increases salt stress tolerance in rice
Comparative study of four rice cultivars with different levels of cadmium tolerance
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice
Overexpression of OsHAD3, a Member of HAD Superfamily, Decreases Drought Tolerance of Rice
Enhanced saccharification of rice straw by overexpression of rice exo-glucanase
Overexpression of OsLCT2, a Low-Affinity Cation Transporter Gene, Reduces Cadmium Accumulation in Shoots and Grains of Rice
A cadmium stress-responsive gene AtFC1 confers plant tolerance to cadmium toxicity
Genetic loci regulating cadmium content in rice grains
Proteomic Analysis of Rice Subjected to Low Light Stress and Overexpression of OsGAPB Increases the Stress Tolerance
Clinical significance of Phosphatidyl Inositol Synthase overexpression in oral cancer