Digital Detection of Single Virus Particles by Multi‐Spot, Label‐Free Imaging Biosensor on Anti‐Reflective Glass
Abstract: Rapid detection of whole virus particles in biological or environmental samples represents an unmet need for the containment of infectious diseases. Here, an optical device enabling the enumeration of single virion particles binding on antibody or aptamers immobilized on a surface with anti‐reflective coating is described. In this regime, nanoparticles adhering to the sensor surface provide localized contributions to the reflected field that become detectable because of their mixing with the interfering waves in the reflection direction. Thus, these settings are exploited to realize a scan‐free, label‐free, micro‐array‐type digital assay on a disposable cartridge, in which the virion counting takes place in wide field‐of‐view imaging. With this approach we could quantify, by enumeration, different variants of SARS‐CoV‐2 virions interacting with antibodies and aptamers immobilized on different spots. For all tested variants, the aptamers showed larger affinity but lower specificity relative to the antibodies. It is found that the combination of different probes on the same surface enables increasing specificity of detection and dynamic range.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Digital Detection of Single Virus Particles by Multi‐Spot, Label‐Free Imaging Biosensor on Anti‐Reflective Glass ; day:14 ; month:04 ; year:2023 ; extent:11
Small ; (14.04.2023) (gesamt 11)
- Creator
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Nava, Giovanni
Casiraghi, Luca
Carzaniga, Thomas
Zanchetta, Giuliano
Chiari, Marcella
Damin, Francesco
Bollati, Valentina
Signorini, Lucia
Delbue, Serena
Bellini, Tommaso
Buscaglia, Marco
- DOI
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10.1002/smll.202300947
- URN
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urn:nbn:de:101:1-2023041515324625666756
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:55 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Nava, Giovanni
- Casiraghi, Luca
- Carzaniga, Thomas
- Zanchetta, Giuliano
- Chiari, Marcella
- Damin, Francesco
- Bollati, Valentina
- Signorini, Lucia
- Delbue, Serena
- Bellini, Tommaso
- Buscaglia, Marco
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