Enrichment of complement, immunoglobulins and autoantibody targets in the proteome of platelets from patients with Systemic Lupus Erythematosus (SLE)

Background SLE is a complex disease characterized by autoimmunity towards apoptotic cells, excessive amounts of circulating immune complexes and complement activation. Decreased platelet size has been observed in SLE and their non-hemostatic functions may play an active role in the disease. Our main objective in this study was to find clues that could explain their decreased size and functional role, analyzing the entire platelet proteome. Methods and patients Platelets were isolated from 23 patients with SLE. The five individuals with highest and lowest average platelet forward scatter were selected for further analysis. Platelet protein content was analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) and compared to platelets from five healthy controls (HC) Data are available via ProteomeXchange with identifier PXD031202. Results Out of 2572 proteins identified, 396 had significantly different levels (ANOVA q-value ≤ 0.01). Forty proteins, including immunoglobulin-, complement- and phosphatidylserine binding proteins had higher abundance in platelets from SLE patients, largely independent of size (fold difference of ≥ 1.5 and a t-test p-value of ≤ 0.05 as cut off). Functional characterization revealed increased degranulation and skewed hemostatic balance in platelets from SLE patients. In the SLE proteome, immunoglobulin proteins were negatively correlated to serum complement C3 and C4 and the highest relative levels was detected in platelets of normal size. Conclusion Platelets from SLE patients shared a specific protein profile, including immunoglobulins, complement proteins and autoantigens, largely independent of platelet size and in agreement with an integrated role for platelets in SLE.

Object Details

• Alternative title: Platelets from SLE patients - enriched in immunoglobulin proteins, complement proteins and auto antigens
• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Enrichment of complement, immunoglobulins and autoantibody targets in the proteome of platelets from patients with Systemic Lupus Erythematosus (SLE) ; day:13 ; month:04 ; year:2022 ; Thrombosis and haemostasis ; (13.04.2022)

Contributors, Places and Time

• Contributor: Linge, Carl Petrus ; Jern, Andreas ; Tydén, Helena ; Gullstrand, Birgitta ; Yan, Hong ; Welinder, Charlotte ; Kahn, Robin ; Jönssen, Andreas ; Semple, John W. ; Bengtsson, Anders A.

Further information

• DOI: 10.1055/a-1825-2915
• URN: urn:nbn:de:101:1-2022060210330044921779
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:24 AM CEST