Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation
Abstract: Spatio‐temporal regulation of signalling pathways plays a key role in generating diverse responses during the development of multicellular organisms. The role of signal dynamics in transferring signalling information in vivo is incompletely understood. Here, we employ genome engineering in Drosophila melanogaster to generate a functional optogenetic allele of the Notch ligand Delta (opto‐Delta), which replaces both copies of the endogenous wild‐type locus. Using clonal analysis, we show that optogenetic activation blocks Notch activation through cis‐inhibition in signal‐receiving cells. Signal perturbation in combination with quantitative analysis of a live transcriptional reporter of Notch pathway activity reveals differential tissue‐ and cell‐scale regulatory modes. While at the tissue‐level the duration of Notch signalling determines the probability with which a cellular response will occur, in individual cells Notch activation acts through a switch‐like mechanism. Thus, time confers regulatory properties to Notch signalling that exhibit integrative digital behaviours during tissue differentiation.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation ; volume:20 ; number:12 ; year:2019 ; extent:13
EMBO reports / European Molecular Biology Organization ; 20, Heft 12 (2019) (gesamt 13)
- Urheber
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Viswanathan, Ranjith
Necakov, Aleksandar
Trylinski, Mateusz
Harish, Rohit Krishnan
Krueger, Daniel
Esposito, Emilia
Schweisguth, Francois
Neveu, Pierre
De Renzis, Stefano
- DOI
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10.15252/embr.201947999
- URN
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urn:nbn:de:101:1-2022081008533376531893
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:32 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Viswanathan, Ranjith
- Necakov, Aleksandar
- Trylinski, Mateusz
- Harish, Rohit Krishnan
- Krueger, Daniel
- Esposito, Emilia
- Schweisguth, Francois
- Neveu, Pierre
- De Renzis, Stefano
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Gene expression analysis reveals that Delta/Notch signalling is not involved in onychophoran segmentation
Regulation of macrophage maturation by notch signalling
Regulation of endothelial Notch signalling by deubiquitinases
The role of Notch signalling in ovarian angiogenesis
Canonical Notch signalling is inactive in urothelial carcinoma
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Gene expression analysis reveals that Delta/Notch signalling is not involved in onychophoran segmentation
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