B1‐type cyclins control microtubule organization during cell division in Arabidopsis
Abstract: Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development‐specific requirements of B1‐type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1‐type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1‐type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA‐TUBULIN COMPLEX PROTEIN 3‐INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1‐type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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EMBO reports. - 23 (2022) , e53995, ISSN: 1469-3178
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
-
Universität
- (wann)
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2024
- Urheber
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Romeiro Motta, Mariana
Zhao, Xin’Ai
Pastuglia, Martine
Belcram, Katia
Roodbarkelari, Farshad
Komaki, Maki
Harashima, Hirofumi
Komaki, Shinichiro
Kumar, Manoj
Bulankova, Petra
Heese, Maren
Riha, Karel
Bouchez, David
Schnittger, Arp
- DOI
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10.15252/embr.202153995
- URN
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urn:nbn:de:bsz:25-freidok-2480758
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:51 MEZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Romeiro Motta, Mariana
- Zhao, Xin’Ai
- Pastuglia, Martine
- Belcram, Katia
- Roodbarkelari, Farshad
- Komaki, Maki
- Harashima, Hirofumi
- Komaki, Shinichiro
- Kumar, Manoj
- Bulankova, Petra
- Heese, Maren
- Riha, Karel
- Bouchez, David
- Schnittger, Arp
- Universität
Entstanden
- 2024
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