An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements †

Abstract: Time series data can provide valuable insight into the complexity of biological reactions. Such information can be obtained by mass‐spectrometry‐based approaches that measure pre‐steady‐state kinetics. These methods are based on a mixing device that rapidly mixes the reactants prior to the on‐line mass measurement of the transient intermediate steps. Here, we describe an improved continuous‐flow mixing apparatus for real‐time electrospray mass spectrometry measurements. Our setup was designed to minimize metal–solution interfaces and provide a sheath flow of nitrogen gas for generating stable and continuous spray that consequently enhances the signal‐to‐noise ratio. Moreover, the device was planned to enable easy mounting onto a mass spectrometer replacing the commercial electrospray ionization source. We demonstrate the performance of our apparatus by monitoring the unfolding reaction of cytochrome C, yielding improved signal‐to‐noise ratio and reduced experimental repeat errors.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: An Improved Rapid Mixing Device for Time‐Resolved Electrospray Mass Spectrometry Measurements † ; volume:3 ; number:3 ; year:2014 ; pages:109-114 ; extent:6 ; ChemistryOpen ; 3, Heft 3 (2014), 109-114 (gesamt 6)

Contributors, Places and Time

• Creator: Zinck, Nicholas ; Stark, Ann-Kathrin ; Wilson, Derek J. ; Sharon, Michal

Further information

• DOI: 10.1002/open.201402002
• URN: urn:nbn:de:101:1-2023011507590832478875
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:21 AM CEST