CdrS is a global transcriptional regulator influencing cell division in Haloferax volcanii
Abstract: Transcriptional regulators that integrate cellular and environmental signals to control cell division are well known in bacteria and eukaryotes, but their existence is poorly understood in archaea. We identified a conserved gene (cdrS) that encodes a small protein and is highly transcribed in the model archaeon Haloferax volcanii. The cdrS gene could not be deleted, but CRISPR interference (CRISPRi)-mediated repression of the cdrS gene caused slow growth and cell division defects and changed the expression of multiple genes and their products associated with cell division, protein degradation, and metabolism. Consistent with this complex regulatory network, overexpression of cdrS inhibited cell division, whereas overexpression of the operon encoding both CdrS and a tubulin-like cell division protein (FtsZ2) stimulated division. Chromatin immunoprecipitation-DNA sequencing (ChIP-Seq) identified 18 DNA-binding sites of the CdrS protein, including one upstream of the promoter for a cell division gene, ftsZ1, and another upstream of the essential gene dacZ, encoding diadenylate cyclase involved in c-di-AMP signaling, which is implicated in the regulation of cell division. These findings suggest that CdrS is a transcription factor that plays a central role in a regulatory network coordinating metabolism and cell division
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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mBio. - 12, 4 (2021) , e01416-21, ISSN: 2150-7511
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2024
- Creator
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Liao, Yan
Vogel, Verena
Hauber, Sabine
Bartel, Jürgen
Alkhnbashi, Omer S.
Maaß, Sandra
Schwarz, Thandi Sarah
Backofen, Rolf
Becher, Dörte
Duggin, Iain G.
Marchfelder, Anita
- DOI
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10.1128/mbio.01416-21
- URN
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urn:nbn:de:bsz:25-freidok-2480011
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:55 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Liao, Yan
- Vogel, Verena
- Hauber, Sabine
- Bartel, Jürgen
- Alkhnbashi, Omer S.
- Maaß, Sandra
- Schwarz, Thandi Sarah
- Backofen, Rolf
- Becher, Dörte
- Duggin, Iain G.
- Marchfelder, Anita
- Universität
Time of origin
- 2024
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