Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis
Abstract: The CRISPR arrays found in many bacteria and most archaea are transcribed into a long precursor RNA that is processed into small clustered regularly interspaced short palindromic repeats (CRISPR) RNAs (crRNAs). These RNA molecules can contain fragments of viral genomes and mediate, together with a set of CRISPR-associated (Cas) proteins, the prokaryotic immunity against viral attacks. CRISPR/Cas systems are diverse and the Cas6 enzymes that process crRNAs vary between different subtypes. We analysed CRISPR/Cas subtype I-B and present the identification of novel Cas6 enzymes from the bacterial and archaeal model organisms Clostridium thermocellum and Methanococcus maripaludis C5. Methanococcus maripaludis Cas6b in vitro activity and specificity was determined. Two complementary catalytic histidine residues were identified. RNA-Seq analyses revealed in vivo crRNA processing sites, crRNA abundance and orientation of CRISPR transcription within these two organisms. Individual spacer sequences were identified with strong effects on transcription and processing patterns of a CRISPR cluster. These effects will need to be considered for the application of CRISPR clusters that are designed to produce synthetic crRNAs
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Nucleic acids research. - 40, 19 (2012) , 9887-9896, ISSN: 1362-4962
- Klassifikation
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Biowissenschaften, Biologie
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2019
- Urheber
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Richter, Hagen Klaus Gunther
Zöphel, Judith
Schermuly, Jeanette
Maticzka, Daniel
Backofen, Rolf
Randau, Lennart
- Beteiligte Personen und Organisationen
- DOI
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10.1093/nar/gks737
- URN
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urn:nbn:de:bsz:25-freidok-1092111
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:46 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Richter, Hagen Klaus Gunther
- Zöphel, Judith
- Schermuly, Jeanette
- Maticzka, Daniel
- Backofen, Rolf
- Randau, Lennart
- Albert-Ludwigs-Universität Freiburg. Professur für Bioinformatik
- Universität
Entstanden
- 2019
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