Mouse Rif1 is a regulatory subunit of protein phosphatase 1 (PP1)

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Abstract: Rif1 is a conserved protein that plays essential roles in orchestrating DNA replication timing, controlling nuclear architecture, telomere length and DNA repair. However, the relationship between these different roles, as well as the molecular basis of Rif1 function is still unclear. The association of Rif1 with insoluble nuclear lamina has thus far hampered exhaustive characterization of the associated protein complexes. We devised a protocol that overcomes this problem, and were thus able to discover a number of novel Rif1 interactors, involved in chromatin metabolism and phosphorylation. Among them, we focus here on PP1. Data from different systems have suggested that Rif1-PP1 interaction is conserved and has important biological roles. Using mutagenesis, NMR, isothermal calorimetry and surface plasmon resonance we demonstrate that Rif1 is a high-affinity PP1 adaptor, able to out-compete the well-established PP1-inhibitor I2 in vitro. Our conclusions have important implications for understanding Rif1 diverse roles and the relationship between the biological processes controlled by Rif1

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Notes: Scientific reports. - 7, 1 (2017) , 2119, ISSN: 2045-2322

Event: Veröffentlichung

(where): Freiburg

(who): Universität

(when): 2021

Creator: Sukackaite, Rasa
Cornacchia, Daniela
Jensen, Malene
Mas, Philippe J.
Blackledge, Martin
Enervald, Elin
Duan, Guangyou
Auchynnikava, Tania
Köhn, Maja
Hart, Darren J.
Buonomo, Sara B. C.

Contributor: Abteilung Integrative Signalforschung

DOI: 10.1038/s41598-017-01910-1

URN: urn:nbn:de:bsz:25-freidok-1947385

Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 10:58 AM CEST

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Associated

Sukackaite, Rasa
Cornacchia, Daniela
Jensen, Malene
Mas, Philippe J.
Blackledge, Martin
Enervald, Elin
Duan, Guangyou
Auchynnikava, Tania
Köhn, Maja
Hart, Darren J.
Buonomo, Sara B. C.
Abteilung Integrative Signalforschung
Universität

Time of origin

2021

Other Objects (12)

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Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Waved with open eyelids 2 (woe2) is a novel spontaneous mouse mutation in the protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l)gene

Protein kinase A regulatory subunit distribution in medulloblastoma

Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb

Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Mapping of the Mouse Actin Capping Protein Beta Subunit Gene

GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Waved with open eyelids 2 (woe2) is a novel spontaneous mouse mutation in the protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l)gene

Protein kinase A regulatory subunit distribution in medulloblastoma

Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb

Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Mapping of the Mouse Actin Capping Protein Beta Subunit Gene

GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

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Mouse Rif1 is a regulatory subunit of protein phosphatase 1 (PP1)

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Other Objects (12)

Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Waved with open eyelids 2 (woe2) is a novel spontaneous mouse mutation in the protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l)gene

Protein kinase A regulatory subunit distribution in medulloblastoma

Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb

Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Mapping of the Mouse Actin Capping Protein Beta Subunit Gene

GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Waved with open eyelids 2 (woe2) is a novel spontaneous mouse mutation in the protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l)gene

Protein kinase A regulatory subunit distribution in medulloblastoma

Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb

Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Mapping of the Mouse Actin Capping Protein Beta Subunit Gene

GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

Insulin-stimulated phosphorylation of protein phosphatase 1 regulatory subunit 12B revealed by HPLC-ESI-MS/MS

Waved with open eyelids 2 (woe2) is a novel spontaneous mouse mutation in the protein phosphatase 1, regulatory (inhibitor) subunit 13 like (Ppp1r13l)gene

Protein kinase A regulatory subunit distribution in medulloblastoma

Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats

A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

PPP1R2 stimulates protein phosphatase-1 through stabilisation of dynamic subunit interactions

Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb

Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]

Mapping of the Mouse Actin Capping Protein Beta Subunit Gene

GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

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