Production of SacI and SacII isoschizomers by soil streptomycetes

Abstract: Five fresh soil Streptomyces spp. strains were isolated, phylogenetically characterized on the basis of 16S rDNA sequences and analyzed for the presence of restriction modification systems. Three type II site-specific endonucleases were detected and partially purified. Two isolated enzymes were isoschizomers of SacI restriction endonuclease recognizing 5′-GAGCTC-3′ sequence; the third one recognised 5′-CCGCGG-3′ sequence and it was an isoschizomer of SacII. SacII like modification was observed in other two isolates having no detectable restriction activity. The lack of correlation between restriction and modification phenotypes and phylogenetic classification of the isolates indicates efficient gene transfer mechanism in the Streptomyces genus.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Production of SacI and SacII isoschizomers by soil streptomycetes ; volume:62 ; number:4 ; year:2007 ; pages:381-385 ; extent:5 ; Biologia ; 62, Heft 4 (2007), 381-385 (gesamt 5)

Contributors, Places and Time

• Creator: Tothova, Timea ; Godany, Andrej ; Javorsky, Peter ; Pristas, Peter

Further information

• DOI: 10.2478/s11756-007-0095-3
• URN: urn:nbn:de:101:1-2409221540005.660320152868
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:23 AM CEST