Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures

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Abstract: Microglia are specialized parenchymal-resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single-cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)-injected mice. By excluding the contribution of other immune CNS-resident and peripheral cells, we show that microglia isolated from LPS-injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease-associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
EMBO reports. - 19, 11 (2018) , e46171, ISSN: 1469-221X

Klassifikation
Medizin, Gesundheit

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2019
Urheber
Sousa, Carole
Golebiewska, Anna
Poovathingal, Suresh K.
Kaoma, Tony
Pires‐Afonso, Yolanda
Martina, Silvia
Coowar, Djalil
Azuaje, Francisco
Skupin, Alexander
Balling, Rudolf
Biber, Knut
Niclou, Simone
Michelucci, Alessandro
Beteiligte Personen und Organisationen

DOI
10.15252/embr.201846171
URN
urn:nbn:de:bsz:25-freidok-1471551
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:28 MESZ

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  • 2019

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