A technology platform for digital nucleic acid diagnostics at the point of care
Abstract: The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, including PCR, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), have already been demonstrated. Our novel approach of integrated sample-to-answer analysis is introduced, and examples for the detection of HIV and single cell analysis of antibiotic resistant bacteria are presented. Next to these LabDisk based systems, a microfluidic cartridge termed DropChip allows for digital amplification using only commercially available laboratory devices
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Laboratoriumsmedizin. - 41, 5 (2017) , ISSN: 0342-3026
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2025
- Creator
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Borst, Nadine
Schuler, Friedrich
Wadle, Simon
Schulz, Martin
Specht, Mara
Li, Jia
Becherer, Lisa
Trotter, Martin
Rodríguez-Martínez, Ana Belén
Paust, Nils
Zengerle, Roland
Stetten, Felix von
- DOI
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10.1515/labmed-2017-0098
- URN
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urn:nbn:de:bsz:25-freidok-1404378
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:23 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Borst, Nadine
- Schuler, Friedrich
- Wadle, Simon
- Schulz, Martin
- Specht, Mara
- Li, Jia
- Becherer, Lisa
- Trotter, Martin
- Rodríguez-Martínez, Ana Belén
- Paust, Nils
- Zengerle, Roland
- Stetten, Felix von
- Universität
Time of origin
- 2025