A technology platform for digital nucleic acid diagnostics at the point of care

Abstract: The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, including PCR, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), have already been demonstrated. Our novel approach of integrated sample-to-answer analysis is introduced, and examples for the detection of HIV and single cell analysis of antibiotic resistant bacteria are presented. Next to these LabDisk based systems, a microfluidic cartridge termed DropChip allows for digital amplification using only commercially available laboratory devices

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch
Notes
Laboratoriumsmedizin. - 41, 5 (2017) , ISSN: 0342-3026

Event
Veröffentlichung
(where)
Freiburg
(who)
Universität
(when)
2025
Creator

DOI
10.1515/labmed-2017-0098
URN
urn:nbn:de:bsz:25-freidok-1404378
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:23 AM CEST

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Time of origin

  • 2025

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