Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis

Abstract: Catalytic activity of catalase (CAT) immobilized on a modified silicate matrix to mediate decomposition of meta-chloroperoxibenzoic acid (3-CPBA) in acetonitrile has been investigated by means of quantitative UV-spectrophotometry. Under the selected experimental conditions, the kinetic parameters: the apparent Michaelis constat (KM), the apparent maximum rate of enzymatic reaction (Vmaxapp), the first order specific rate constants (ksp), the energy of activation (Ea) and the pre-exponential factor of the Arrhenius equation (Z0) were calculated. Conclusions regarding the rate-limiting step of the overall catalytic process were drawn from the calculated values of the Gibbs energy of activation ΔG*, the enthalpy of activation ΔH*, and the entropy of activation ΔS*.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis ; volume:3 ; number:2 ; year:2005 ; pages:279-287 ; extent:9 ; Open chemistry ; 3, Heft 2 (2005), 279-287 (gesamt 9)

Beteiligte, Orts- und Zeitangaben

• Urheber: Horozova, Elena ; Dimcheva, Nina

Weitere Informationen

• DOI: 10.2478/BF02475996
• URN: urn:nbn:de:101:1-2410171647245.034373033039
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:24 MESZ