Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices

Abstract: A novel ultra‐low‐cost biochemical analysis platform to quantify protein dissociation binding constants and kinetics using paper microfluidics is reported. This approach marries video imaging with one of humankind's oldest materials: paper, requiring no large, expensive laboratory equipment, complex microfluidics or external power. Temporal measurements of nanoparticle–antibody conjugates binding on paper is found to follow the Langmuir Adsorption Model. This is exploited to measure a series of antibody–antigen dissociation constants on paper, showing excellent agreement with a gold‐standard benchtop interferometer. The concept is demonstrated with a camera and low‐end smartphone, 500‐fold cheaper than the reference method, and can be multiplexed to measure ten reactions in parallel. These findings will help to widen access to quantitative analytical biochemistry, for diverse applications spanning disease diagnostics, drug discovery, and environmental analysis in resource‐limited settings.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Quantifying Biomolecular Binding Constants using Video Paper Analytical Devices ; volume:24 ; number:39 ; year:2018 ; pages:9783-9787 ; extent:5 ; Chemistry - a European journal ; 24, Heft 39 (2018), 9783-9787 (gesamt 5)

Contributors, Places and Time

• Creator: Miller, Benjamin S. ; Parolo, Claudio ; Turbé, Valérian ; Keane, Candice E. ; Gray, Eleanor R. ; McKendry, Rachel A.

Further information

• DOI: 10.1002/chem.201802394
• URN: urn:nbn:de:101:1-2022082105475784588043
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:20 AM CEST