Optical and Electrochemical Probes for Monitoring Cytochrome c in Subcellular Compartments During Apoptosis
Abstract: Cytochrome c (Cyt. c) is a key initiator of the caspases that activate cell apoptosis. The spatiotemporal evaluation of the contents of Cyt. c in cellular compartments and the detection of Cyt. c delivery between cellular compartments upon apoptosis is important for probing cell viabilities. We introduce an optical probe and an electrochemical probe for the quantitative assessment of Cyt. c in cellular compartments at the single cell level. The optical or electrochemical probes are functionalized with photoresponsive o‐nitrobenzylphosphate ester‐caged Cyt. c aptamer constituents. These are uncaged by light stimuli at single cell compartments, allowing the spatiotemporal detection of Cyt. c through the formation of Cyt. c/aptamer complexes at non‐apoptotic or apoptotic conditions. The probes are applied to distinguish the contents of Cyt. c in cellular compartments of epithelial MCF‐10A breast cells and malignant MCF‐7 and MDA‐MB‐231 breast cells under apoptotic/non‐apoptotic conditions.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Optical and Electrochemical Probes for Monitoring Cytochrome c in Subcellular Compartments During Apoptosis ; day:27 ; month:03 ; year:2023 ; extent:11
Angewandte Chemie / International edition. International edition ; (27.03.2023) (gesamt 11)
- Creator
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Duan, Zhijuan
Ouyang, Yu
Fu, Yuzhe
Huang, Fujian
Xia, Fan
Willner, Itamar
- DOI
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10.1002/anie.202301476
- URN
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urn:nbn:de:101:1-2023032815054440048158
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:47 AM CEST
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Associated
- Duan, Zhijuan
- Ouyang, Yu
- Fu, Yuzhe
- Huang, Fujian
- Xia, Fan
- Willner, Itamar
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Redox-poise between subcellular compartments in plant cells
Brillouin microscopy monitors rapid responses in subcellular compartments
Quantitative calcium measurements in subcellular compartments of Plasmodium falciparum-infected erythrocytes
Molecular view on the spatiotemporal organization of Bacillus subtilis subcellular compartments
Calculation of the relative metastabilities of proteins in subcellular compartments of Saccharomyces cerevisiae
Multiscale Asymptotic Analysis Reveals How Cell Growth and Subcellular Compartments Affect Tissue-Scale Hormone Transport
Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments
Integration of Organic Electrochemical Transistors with Implantable Probes
Real-time imaging of the glutathione redox potential in subcellular compartments of the malaria parasite Plasmodium falciparum
Environmental compartments, Buch.